期刊在線咨詢(xún)服務(wù)，立即咨詢(xún)
購(gòu)物車(chē)(0)
OA-4 Thioredoxin-1 and Geranylgeranylacetone Resist Neurotoxicity of Amyloid-β
關(guān)鍵詞：disease cognitive function
摘要：Objective:Alzheimer's disease (AD) is the most common neurodegenerative disorder which is characterized by amyloid-β( Aβ) aggradation in the brain and impairment of cognitive function. Thioredoxin-1( Trx-1) is a redox regulating protein,and plays roles in resisting the oxidative stress and protecting neurons. Our previous study found that Trx-1 improved the cognitive function of Parkinson's Disease (PD) mice. Geranylgeranylacetone (GGA) is an antiulcer drug and induces the expression of Trx-1 in vivo and in vitro. However,whether Trx-1 improves cognitive functions in mice of APP/PS1 or GGA protects SH-SY5Y cells from cytotoxicity induced by Aβ is still unknown. The objective of present is to investigate the roles of Trx-1 and GGA in inhibiting neurotoxicity of Aβ. Methods:We used MTT assay to test the cell viability induced by Aβ(25-35) and western blot to detect the expression of Trx-1 in SH-SY5Y cells. Trx-1 overexpression transgenic mice were hybridized with APP/PS1 transgenic mice to get control,Trx-1,Tx-1/APP/PS1 and APP/PS1 mice. Then we used Morris water maze,high plus maze and object recognition test to detect the cognitive function of different kinds of mice. We also used RT-PCR and western blot to test the mRNA level and expression of Trx-1,APP,PS1 and Aβ. Results:In our present study,we demonstrated that Aβ(25-35) decreased the cell viability and the expression of Trx-1 in SH-SY5Y cells. The cell viability and the expression of Trx-1 were reversed by GGA. Our results showed that the escape latency in APP/PS1 mice was longer when compared with the Trx-1/APP/PS1 mice in Morris water maze and high plus maze. Whereas navigational experiments in Morris water maze result showed that the total number of crossings and the percentage of time spent in the target quadrant were significantly decreased in APP/PS1 mice when compared to Trx-1/APP/PS1 mice. Object recognition 1/APP/PS1 mice. The mRNA levels and the expression of APP,PS1 and Aβ were decreased in Trx- 1/APP/PS1 mice when compared to APP/PS1 mice
神經(jīng)藥理學(xué)報(bào)雜志要求:
{1}包括真實(shí)姓名、性別、出生年、職務(wù)職稱(chēng)、學(xué)位、研究方向、聯(lián)系電話(huà)、E-mail、郵編等。
{2}文稿應(yīng)論點(diǎn)明確、資料可靠、數(shù)據(jù)精確、文字精煉、層次清晰。
{3}年份中的前兩位數(shù)不可省略，如：‘'01年”應(yīng)為“2001年”。
{4}注釋?zhuān)褐饕糜趯?duì)文章篇名、作者及文內(nèi)某一專(zhuān)題內(nèi)容作必要的解釋或說(shuō)明。序號(hào)在注釋處右上角用阿拉伯?dāng)?shù)字①②等表示。
{5}正文應(yīng)盡量減少層次，一般不宜超過(guò)3層。有標(biāo)題內(nèi)容才列數(shù)字序號(hào)（沒(méi)有標(biāo)題的內(nèi)容用自然段形式起始），數(shù)字序號(hào)后不用加點(diǎn)，但要空1格。
注：因版權(quán)方要求，不能公開(kāi)全文，如需全文，請(qǐng)咨詢(xún)雜志社
